cdh1 cdna in pcdna3 Search Results


cdh1 cdna in pcdna3  (Addgene inc)
92
Addgene inc cdh1 cdna in pcdna3
Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Cdh1 Cdna In Pcdna3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cdh1 pcdna3 plasmid  (Addgene inc)
92
Addgene inc human cdh1 pcdna3 plasmid
Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Human Cdh1 Pcdna3 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdh1+cdna+in+pcdna3/pCDNA3-human+C-MYC-V394D+(Plasmid+%2374162)/pm29742177-209-0-6
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tnt rabbit reticulolysate kit  (Promega)
90
Promega tnt rabbit reticulolysate kit
Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Tnt Rabbit Reticulolysate Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 ha  (Addgene inc)
93
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Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Pcdna3 Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag hcrm1  (Addgene inc)
93
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Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Flag Hcrm1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prk5 fyn  (Addgene inc)
93
Addgene inc prk5 fyn
Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Prk5 Fyn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lenticrisprv2  (Addgene inc)
96
Addgene inc lenticrisprv2
Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pdonr223 src  (Addgene inc)
93
Addgene inc pdonr223 src
Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Pdonr223 Src, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pdonr223 yes1  (Addgene inc)
93
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Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Pdonr223 Yes1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pdonr223 syk  (Addgene inc)
90
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Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
Pdonr223 Syk, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 e2f4  (Addgene inc)
93
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Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
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plenti pgk hygro dest  (Addgene inc)
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Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression <t>of</t> <t>E-cadherin</t> protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.
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Image Search Results


Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression of E-cadherin protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.

Journal: Cancer Research

Article Title: A Runaway PRH/HHEX-Notch3–Positive Feedback Loop Drives Cholangiocarcinoma and Determines Response to CDK4/6 Inhibition

doi: 10.1158/0008-5472.can-19-0942

Figure Lengend Snippet: Figure 2. Effects of PRH manipulation on CCA cell biology. A, Proliferation of CCLP1 cells stably transfected with PRH shRNA or scrambled control; n ¼ 3; P ¼ 0.03. B, Proliferation of CCLP1 and CCSW1 cells infected with Ad myc-PRH or empty virus control; n ¼ 3; P ¼ 0.03 (CCLP1); P ¼ 0.02 (CCSW1). C, Proliferation of AKN1 and primary BECs overexpressing GFP-PRH-myc (stable) or myc-PRH (transient, 48 hours); n ¼ 3; P ¼ 0.003 (AKN1); P ¼ 0.006 (BEC). D, Morphology of CCLP1 cells stably transfected with PRH shRNA or scrambled control. E, Western blot showing increased expression of E-cadherin protein and decreased expression of vimentin protein after PRH knockdown. Lamin A/C was used as loading control. F, Migration of CCLP1 PRH knockdown cells through transwell filters in a 10% serum gradient; n ¼ 3; P ¼ 0.03. G, Invasion of CCLP1 cells through Matrigel; n ¼ 4; P ¼ 0.03. H, Western blotting for myc-PRH and EMT-associated proteins E-cadherin and vimentin in AKN1 cells and primary BECs. I and J, As in F and G for AKN1 cells and primary BECs. K, Morphology of equal numbers of AKN1 cells stably transfected with plasmids expressing GFP (control) or a GFP-PRH-Myc–tagged fusion protein (GFP-PRH-Myc). Scale bars, 50 mm in length. , P < 0.05.

Article Snippet: Stable cell lines were generated by transfecting PRH shRNA in pRS (Origene, TR312464), Notch3 shRNA in pLKO (Sigma-Aldrich, TRCN0000363316), CDH1 cDNA in pCDNA3 (hE-cadherinpcDNA3 was a gift from Dr. Barry Gumbiner, Seattle Children's Hospital, Seattle, WA; Addgene plasmid #45769), or EGFP-PRHmyc in pEGFP-C1.

Techniques: Stable Transfection, Transfection, shRNA, Control, Infection, Virus, Western Blot, Expressing, Knockdown, Migration

Figure 4. Notch3 expression is regulated by PRH. A, Western blot showing Notch3 ICD protein expression in PRH knockdown CCLP1 cells. B, NOTCH3 gene expression in CCLP1 and CCSW1 cells infected with Ad myc-PRH, Ad myc-PRH DNA-binding deficient N187A mutant, or empty virus control. C, Western blot for CCLP1 samples in B. D, Notch3 ICD rotein expression correlates with PRH protein expression in four human CCA cell lines, two human primary BEC isolates, and an immortalized BEC line. E, Western blot showing elevated expression of Notch3 ICD upon PRH overexpression in both AKN1 cells and primary BECs. F, Western blot showing increased expression of E-cadherin and reduced expression of PRH proteins after Notch3 knockdown. Lamin A/C was used as loading control. G, Proliferation of CCLP1 cells stably transfected with Notch3 shRNA or scrambled control; n ¼ 3; P ¼ 0.04. H, Morphology of CCLP1 cells stably transfected with Notch3 shRNA or scrambled control. I, DEGs detected in Notch3 KD compared with PRH KD experiments. Hypergeometric test, P ¼ 10229 for upregulated genes and P ¼ 1031 for downregulated genes. J, Hallmark GSEA of genes differentially expressed after Notch3 KD. Red bars, gene sets that are also enriched after PRH knockdown. , P < 0.05.

Journal: Cancer Research

Article Title: A Runaway PRH/HHEX-Notch3–Positive Feedback Loop Drives Cholangiocarcinoma and Determines Response to CDK4/6 Inhibition

doi: 10.1158/0008-5472.can-19-0942

Figure Lengend Snippet: Figure 4. Notch3 expression is regulated by PRH. A, Western blot showing Notch3 ICD protein expression in PRH knockdown CCLP1 cells. B, NOTCH3 gene expression in CCLP1 and CCSW1 cells infected with Ad myc-PRH, Ad myc-PRH DNA-binding deficient N187A mutant, or empty virus control. C, Western blot for CCLP1 samples in B. D, Notch3 ICD rotein expression correlates with PRH protein expression in four human CCA cell lines, two human primary BEC isolates, and an immortalized BEC line. E, Western blot showing elevated expression of Notch3 ICD upon PRH overexpression in both AKN1 cells and primary BECs. F, Western blot showing increased expression of E-cadherin and reduced expression of PRH proteins after Notch3 knockdown. Lamin A/C was used as loading control. G, Proliferation of CCLP1 cells stably transfected with Notch3 shRNA or scrambled control; n ¼ 3; P ¼ 0.04. H, Morphology of CCLP1 cells stably transfected with Notch3 shRNA or scrambled control. I, DEGs detected in Notch3 KD compared with PRH KD experiments. Hypergeometric test, P ¼ 10229 for upregulated genes and P ¼ 1031 for downregulated genes. J, Hallmark GSEA of genes differentially expressed after Notch3 KD. Red bars, gene sets that are also enriched after PRH knockdown. , P < 0.05.

Article Snippet: Stable cell lines were generated by transfecting PRH shRNA in pRS (Origene, TR312464), Notch3 shRNA in pLKO (Sigma-Aldrich, TRCN0000363316), CDH1 cDNA in pCDNA3 (hE-cadherinpcDNA3 was a gift from Dr. Barry Gumbiner, Seattle Children's Hospital, Seattle, WA; Addgene plasmid #45769), or EGFP-PRHmyc in pEGFP-C1.

Techniques: Expressing, Western Blot, Knockdown, Gene Expression, Infection, Binding Assay, Mutagenesis, Virus, Control, Over Expression, Stable Transfection, Transfection, shRNA

Figure 5. Notch3- and PRH-correlated gene sets. A, qRT-PCR analysis of genes from CCLP1 cells overexpressing PRH in the presence or absence of Notch3 shRNA identifying PRH and Notch3-correlated expression signatures. B, Western blot analysis of EMT proteins E-cadherin and vimentin and cyclin D2 in CCLP1 cells overexpressing myc-PRH in the presence of absence of Notch3 shRNA. C, Proliferation of CCLP1 cells overexpressing myc-PRH in the presence or absence of Notch3 shRNA. , P < 0.05 after Bonferroni correction, compared with nontargeting shRNA/empty virus control. #, no statistically significant difference in the comparison indicated. D, Hallmark GSEA of Notch3-correlated and PRH-correlated gene sets identified from analysis of RNA-seq data. Red bars, gene sets enriched in both PRH- and Notch3-correlated sets. E, Overlap of genes with PRH-binding sites determined by ChIP-seq and genes that are differentially expressed after PRH overexpression determined by RNA-seq in CCLP1 cells. F, Comparison of the primary motif underlying PRH ChIP-seq peaks identified using HOMER with the PRH SELEX motif (derived from ref. 38). G, RNA-seq and ChIP-seq tracks of putative direct PRH target DKK1. Red tracks, myc-PRH overexpression. H, RNA-seq and ChIP-seq tracks of putative direct PRH target WNT11. Red tracks, myc-PRH overexpression.

Journal: Cancer Research

Article Title: A Runaway PRH/HHEX-Notch3–Positive Feedback Loop Drives Cholangiocarcinoma and Determines Response to CDK4/6 Inhibition

doi: 10.1158/0008-5472.can-19-0942

Figure Lengend Snippet: Figure 5. Notch3- and PRH-correlated gene sets. A, qRT-PCR analysis of genes from CCLP1 cells overexpressing PRH in the presence or absence of Notch3 shRNA identifying PRH and Notch3-correlated expression signatures. B, Western blot analysis of EMT proteins E-cadherin and vimentin and cyclin D2 in CCLP1 cells overexpressing myc-PRH in the presence of absence of Notch3 shRNA. C, Proliferation of CCLP1 cells overexpressing myc-PRH in the presence or absence of Notch3 shRNA. , P < 0.05 after Bonferroni correction, compared with nontargeting shRNA/empty virus control. #, no statistically significant difference in the comparison indicated. D, Hallmark GSEA of Notch3-correlated and PRH-correlated gene sets identified from analysis of RNA-seq data. Red bars, gene sets enriched in both PRH- and Notch3-correlated sets. E, Overlap of genes with PRH-binding sites determined by ChIP-seq and genes that are differentially expressed after PRH overexpression determined by RNA-seq in CCLP1 cells. F, Comparison of the primary motif underlying PRH ChIP-seq peaks identified using HOMER with the PRH SELEX motif (derived from ref. 38). G, RNA-seq and ChIP-seq tracks of putative direct PRH target DKK1. Red tracks, myc-PRH overexpression. H, RNA-seq and ChIP-seq tracks of putative direct PRH target WNT11. Red tracks, myc-PRH overexpression.

Article Snippet: Stable cell lines were generated by transfecting PRH shRNA in pRS (Origene, TR312464), Notch3 shRNA in pLKO (Sigma-Aldrich, TRCN0000363316), CDH1 cDNA in pCDNA3 (hE-cadherinpcDNA3 was a gift from Dr. Barry Gumbiner, Seattle Children's Hospital, Seattle, WA; Addgene plasmid #45769), or EGFP-PRHmyc in pEGFP-C1.

Techniques: Quantitative RT-PCR, shRNA, Expressing, Western Blot, Virus, Control, Comparison, RNA Sequencing, Binding Assay, ChIP-sequencing, Over Expression, Derivative Assay

Figure 6. Regulation of Wnt signaling. A, TOPFlash TCF/LEF reporter activity in CCLP1 and CCSW1 cells expressing myc-PRH, DNA-binding–deficient N187A mutant of myc- PRH and Flag-Notch3-ICD. Inset, Western blot showing expression of myc-PRH constructs. B, Representative immunofluorescence micrographs of CCLP1 PRH knockdown cells stained for E-cadherin and b-catenin. C, Western blot of subcellular fractions of CCLP1 PRH knockdown cells. D, TOPFlash reporter activity in PRH knockdown CCLP1 cells. E, TOPFlash reporter activity in control CCLP1 cells (pcDNA empty) and CCLP1 cells overexpressing E-cadherin (CDH1) in the presence and absence of myc-PRH expression. Inset, Western blot showing successful overexpression of E-cadherin. , P < 0.05; , P < 0.01; #, no statistically significant difference.

Journal: Cancer Research

Article Title: A Runaway PRH/HHEX-Notch3–Positive Feedback Loop Drives Cholangiocarcinoma and Determines Response to CDK4/6 Inhibition

doi: 10.1158/0008-5472.can-19-0942

Figure Lengend Snippet: Figure 6. Regulation of Wnt signaling. A, TOPFlash TCF/LEF reporter activity in CCLP1 and CCSW1 cells expressing myc-PRH, DNA-binding–deficient N187A mutant of myc- PRH and Flag-Notch3-ICD. Inset, Western blot showing expression of myc-PRH constructs. B, Representative immunofluorescence micrographs of CCLP1 PRH knockdown cells stained for E-cadherin and b-catenin. C, Western blot of subcellular fractions of CCLP1 PRH knockdown cells. D, TOPFlash reporter activity in PRH knockdown CCLP1 cells. E, TOPFlash reporter activity in control CCLP1 cells (pcDNA empty) and CCLP1 cells overexpressing E-cadherin (CDH1) in the presence and absence of myc-PRH expression. Inset, Western blot showing successful overexpression of E-cadherin. , P < 0.05; , P < 0.01; #, no statistically significant difference.

Article Snippet: Stable cell lines were generated by transfecting PRH shRNA in pRS (Origene, TR312464), Notch3 shRNA in pLKO (Sigma-Aldrich, TRCN0000363316), CDH1 cDNA in pCDNA3 (hE-cadherinpcDNA3 was a gift from Dr. Barry Gumbiner, Seattle Children's Hospital, Seattle, WA; Addgene plasmid #45769), or EGFP-PRHmyc in pEGFP-C1.

Techniques: Activity Assay, Expressing, Binding Assay, Mutagenesis, Western Blot, Construct, Knockdown, Staining, Control, Over Expression